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ability again along artificial chain copies critical cut designing easily engineer genes millions paste pieces process putting rapidly sites specific spots together using
To make use of artificial genes we need to make millions of copies of them for experiments using a process called polymerase chain reaction. By putting restriction sites into specific spots along the gene, we can cut it into bite-sized pieces that are easily duplicated millions of times. So the ability to cut and paste genes back together again is critical for designing genes to the right specifications, rapidly replicating them and putting them into vectors to genetically engineer cells. Jef Boeke
ability again along artificial chain copies critical cut designing easily engineer genes millions paste pieces process putting rapidly sites specific spots together using
To make use of artificial genes, we need to make millions of copies of them for experiments using a process called polymerase chain reaction. By putting restriction sites into specific spots along the gene, we can cut it into bite-sized pieces that are easily duplicated millions of times. So the ability to cut and paste genes back together again is critical for designing genes to the right specifications, rapidly replicating them and putting them into vectors to genetically engineer cells.
ability came everybody football plays terrific
Everybody got their money's worth. It was a terrific football game. It came down to our ability to make some plays in the end. Marty Schottenheimer
ability applaud aspects children concerned customers demand exploit helping hopefully horrific increases pimps pioneering sexual sexually terrible toward ways women
Everyone who is concerned about the sexual victimization of children and women should applaud the enactment of this legislation. It significantly increases the ability of authorities to go after the pimps and the customers who exploit these young women and children sexually and in such horrific ways. The so-called End Demand aspects of this legislation are pioneering in many ways and hopefully will go a long way toward helping to end the terrible sexual exploitation going on in our nation. Richard Land
ability based cognitive form mental specific
Based on her cognitive deficits, yes, she lacked the mental capacity, the cognitive ability to form the specific intent,
ability accelerate beginning consumers continue economic false funk hopes pace raising relative remain tend
At the end of a long economic expansion, consumers tend to be overconfident relative to their spending; raising false hopes about the ability of consumers to continue spending. At the beginning of a recovery, consumers remain in a funk even as they accelerate their pace of spending.
ability american-artist animated business draw guys living mostly newspaper people
At that time, the people that were in the animated film business were mostly guys who were unsuccessful newspaper cartoonists. In other words, their ability to draw living things was practically nil.
ability communication customer designed drive employee employees focus increase increased multimedia possible providing responsive results solutions work worker
At Nortel, our focus is providing multimedia communication capabilities that increase employee productivity and drive innovation, ... Our new IP multimedia solutions are designed to make it possible for employees to collaborate and work more effectively. The results are a more collaborative environment, increased worker productivity and the ability to be much more responsive to customer needs.
ability athletes attend community continue create economic excited explore forum girls global help models positive role serve social support throughout ways women
Women athletes have the ability to serve as role models for women and girls throughout the world. I am excited to attend the World Economic Forum to explore ways in which the global community can continue to support athletes? ability to help create positive social change. Monica Seles
eight huge nice playing pressure win wins worry
That was a huge win for us. It is nice to take some of the pressure of getting eight wins off our back. Now we just have to worry about playing well and getting healthy.
eight happy needed three
That's what I needed to do, ... I made eight birdies ... and, unfortunately, three bogeys. So I'm still making mistakes. But I've got to be happy with that. Ernie Els
eight happy kids top
I was really happy with the boys. We had eight kids in the top 15 and that to me is just awesome.
eight send
I wasn't going to send him back out for eight more pitches.
eight feet kick last lay needed practicing tired until
I wasn't tired until we got done; you're just going through so much. I think I'm just going to go lay down and kick my feet up. I think I did all the practicing I needed to do on those last eight holes.
eight giants hard jackie latino players realize signed since talk
I would never have thought that would happen, ... Remember, when I signed with the Giants in 1955, it was only eight years since Jackie Robinson. But when I talk to Latino players today, a lot of them don't even realize how hard it was for us then. Orlando Cepeda
eight feet help knock level needs next points within
Most of his points have come within eight feet of the basket. He just needs to knock some more jumpers down consistently. He hasn't had to for us, but that's something that at the next level is going to help him a lot.
eight exciting finishing games hanging home learn scoring talked team
We have only eight home games all season, so we've had to learn to play on the road. We're not scoring much, but we're hanging around (the .500 mark). It would be really exciting for such an inexperienced team to make the tourney. We've talked about finishing strong.
eight shoot
We have one through eight that can shoot even or under (par). Greg Murphy
hope people percent town
Probably 75 percent of the people in this town think I'll fail, and the other 25 percent hope I fail, Toby Keith
hope military gentleman
PRIVATE, n. A military gentleman with a field-marshal's baton in his knapsack and an impediment in his hope. Ambrose Bierce
hope mean poor
If I ever get real rich, I hope I'm not real mean to poor people, like I am now
hope life
I hope life isn't a big joke, because I don't get it.
hope interest sign
I hope very much the Serbs will see it in their interest also to sign on,
hope
I hope very much that there aren't other victims. I just don't know at this point.
hope run toward
I hope to run toward the end of the season,
hope people prove wrong
I hope to prove these people wrong very, very quickly.
hope kidnapped prevent sexually somebody students suppose worst
I hope to prevent one of out students from getting sexually assaulted, kidnapped or I suppose the worst would be somebody panics and kills somebody.
kids play oil
As a kid I'd play with homemade recipes, like putting pineapple on my face to exfoliate my skin and doing facial steams with lavender or peppermint oils. I just loved doing stuff like that. It's what motivated me to launch my skin care line. Demi Lovato
kids passion space
As a kid, I wanted to be an astronaut. And my own passion was that I wanted to be a film director. I realized that being an astronaut was not going to be an option, so I said, "Well, I'm going to be a director and do films in space." Alfonso Cuaron
kids technology law
A lot of the films I like are more than fantasies - they're movies fascinated by the technology of space exploration, and they try to honor the laws of physics. I watched the Gregory Peck movie 'Marooned' over and over when I was a kid.
kids people want
When you work with kids, people tell you to be very delicate, but that's the last thing you should do with kids. They feel patronized if you're like that. They just want you to be normal.
kids feelings important
How we feel about our kids isn't as important as how they experience those feelings and how they regard the way we treat them. Alfie Kohn
kids thinking land
America always seemed to me this foreign land that I imagined I could escape to if I needed to get away - and I think that came both from the fact that I was born there and from watching so many American movies when I was a kid. I was brought up on American films. Andrew Garfield
kids men tough-times
As an adolescent, Spider-Man was what got me through tough times in terms of being a skinny kid.
kids winning games
We challenge our kids to be close around the 2:00 mark. If we can do that we can win games. Amy Stephens
kids rocks my-best-friend
I feel like you know what you're going to be good at when you're older based on what you like when you're younger. When I was younger my best friend was Tony, this kid Tony, and he loved rocks. He was always playing with rocks, counting them, and now he's a crack head. Amy Schumer
leadership basketball practice
I just love the game of basketball so much. The game! I don't need the 18,000 people screaming and all the peripheral things. To me, the most enjoyable part is the practice and preparation. Bobby Knight
leadership business crazy
When trouble arises and things look bad, there is always one individual who perceives a solution and is willing to take command. Very often, that individual is crazy. Dave Barry
leadership steps arrogant
God will never direct us to be prideful, arrogant and unforgiving, immoral or slothful or full of fear. We step into these things because we are insensitive to the leadership of the Holy Spirit within us. Charles Stanley
leadership decision benefits
Leadership requires the courage to make decisions that will benefit the next generation. Alan Autry
leadership sports winning
So what if I never win my fifth gold medal; It's only one end of the string. It's competing that matters. It's proving that there is a place for guys like me in sports. It's a persona challenge to extend myself. Al Oerter
leadership basketball sports
Winning is overrated. The only time it is really important is in surgery and war. Al McGuire
leadership sports zero
I believe in a business boarding up early. If you make a mistake, you put the boards in the window of the store and say, "Hey, I made a mistake." Let me take two shots in the arm and a punch on the nose and let me get on to the next thing. I don't believe in worrying over failures. I worry about successes. This is opposite from most people. Most people zero in on their failures. I try to keep all my attention on a pyramid type philosophy rather than the averaging-down philosophy.
leadership voice ideals
The ideal leader is one who hears the voice of God, and beckons on as the voice calls him and them. Aiden Wilson Tozer
leadership work reality
I would rather die than be in the United States Senate. I would be bored to death. Could you imagine me, banging around that chamber with 99 other people, asking for a motion on the amendment in the subcommittee? Forget it...You'd watch me just walk out and walk right into the Potomac River and drown. That would be it. Chris Christie
likes attorney jurisdiction
John [Mulaney] likes to say I'm like a district attorney with absolutely no jurisdiction whatsoever. Nasim Pedrad
national persuaded reform trying unwilling
We've become persuaded that, after two years of trying to reform the national AFL-CIO, it is unwilling to change,
reading practice scripts
I practice reading all the time. I read everything and having so many scripts to read, which really helps out as well. Bella Thorne
reading writing thinking
Close reading of tough-minded writing is still the best, cheapest, and quickest method known for learning to think for yourself... Reading, and rigorous discussion of that reading in a way that obliges you to formulate a position and support it against objections, is an operational definition of education... reading, analysis, and discussion is the way we develop reliable judgment, the principle way we come to penetrate covert movements behind the facade of public appearances. John Taylor Gatto
reading writing different
Being a librarian certainly helped me with my writing because it made me even more of a reader, and I was always an enthusiastic reader. Writing and reading seem to me to be different aspects of a single imaginative act. Margaret Mahy
reading soul rendezvous
Reading is a rendezvous with your soul. Jeanette Winterson
reading writing trying
Writing is a way of life. I do it because it's a way of experiencing the world, and trying to come to terms with it and understand it and express it and engage with it. And to me it's a natural extension of reading. Emily Perkins
reading vision done
Truthfully, in the beginning [of MacGyver] this could have gone either way, and as it turned out there was a version that was done wrong, which I'm not even going to get into. It was a pretty good idea and I liked where it was going, but then we got a chance to restart with Peter Lenkov [as executive producer/showrunner], who brought his vision to it. I remember reading his pilot script and it was just so exciting that I started hopping around my room. Lucas Till
reading hook pages
It's a comedy thriller, brilliantly written and it's full of twists and turns at every page. When I was reading it I was desperate to get to the end to find out what happens, it really hooks you. Louise Jameson
reading yale rome
Great lecturers seldom hesitate to use dramatic tricks to enshrine their precepts in the minds of their audiences, and at Yale perhaps Chauncey B. Tinker was the most noted. To read one of his lectures was like reading a monologue of the great actress Ruth Draper--you missed the main point. You missed the drop in his voice as he approached the death in Rome of the tubercular Keats; you missed the shaking tone in which he described the poet's agony for the absent Fanny with him his love had never been consummated; you missed the grim silence of the end. Louis Auchincloss
reading knowledge answers
One of the pleasures of reading old letters is the knowledge that they need no answer. Lord Byron
skills littles sticks
Learning to shoot firearms to me is a little like driving stick - it seems like a decent skill to have. Sarah Wayne Callies
team winning texas
During my 40-year coaching career at West Point, Indiana and Texas Tech, my teams reached the Final Four on five occasions, winning the national championship three times.
team successful leader
To be successful in coaching you have to treat your team like a family. The leader needs backing from everyone. Morgan Wootten
team ties houston
I still have very close ties to Houston and the Astros because that was my first team. Joe Morgan
team games effort
I've figured out that life in general is a team effort; it's a team game. Joe Namath
team struggle kissing
I wanna kiss you. I couldn't care less about the team struggling. What we know is we can improve. Chad Pennington, our quarterback, missed the first part of the season, and we struggled. We're looking to next season. We're looking to make a noise now, and I wanna kiss you!
team winning six
When you have five or six potential hall of famers on your team ... that's when you win penants and championships. The only thing you have to be concerned about is getting the ballplayers to bed on time. Joe DiMaggio
team good-person persons
I'm a good person but a shitty writer. You're a shitty person but a good writer. We'd make a good team. John Green
team cocky hockey
Hopefully someone on their team addresses it, because, uh, I'm not saying I'm going to do it, but something might happen to him if he continues to be that cocky. Mike Richards
team careers impact
I tell people in their careers, 'Look for growth. Look for the teams that are growing quickly. Look for the companies that are doing well. Look for a place where you feel that you can have a lot of impact.' Sheryl Sandberg