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ability again along artificial chain copies critical cut designing easily engineer genes millions paste pieces process putting rapidly sites specific spots together using
To make use of artificial genes we need to make millions of copies of them for experiments using a process called polymerase chain reaction. By putting restriction sites into specific spots along the gene, we can cut it into bite-sized pieces that are easily duplicated millions of times. So the ability to cut and paste genes back together again is critical for designing genes to the right specifications, rapidly replicating them and putting them into vectors to genetically engineer cells. Jef Boeke
ability again along artificial chain copies critical cut designing easily engineer genes millions paste pieces process putting rapidly sites specific spots together using
To make use of artificial genes, we need to make millions of copies of them for experiments using a process called polymerase chain reaction. By putting restriction sites into specific spots along the gene, we can cut it into bite-sized pieces that are easily duplicated millions of times. So the ability to cut and paste genes back together again is critical for designing genes to the right specifications, rapidly replicating them and putting them into vectors to genetically engineer cells. Jef Boeke
ability came everybody football plays terrific
Everybody got their money's worth. It was a terrific football game. It came down to our ability to make some plays in the end. Marty Schottenheimer
ability based cognitive form mental specific
Based on her cognitive deficits, yes, she lacked the mental capacity, the cognitive ability to form the specific intent, Jonathan Crisp
ability accelerate beginning consumers continue economic false funk hopes pace raising relative remain tend
At the end of a long economic expansion, consumers tend to be overconfident relative to their spending; raising false hopes about the ability of consumers to continue spending. At the beginning of a recovery, consumers remain in a funk even as they accelerate their pace of spending. Carl Steidtmann
ability american-artist animated business draw guys living mostly newspaper people
At that time, the people that were in the animated film business were mostly guys who were unsuccessful newspaper cartoonists. In other words, their ability to draw living things was practically nil. Marc Davis
ability communication customer designed drive employee employees focus increase increased multimedia possible providing responsive results solutions work worker
At Nortel, our focus is providing multimedia communication capabilities that increase employee productivity and drive innovation, ... Our new IP multimedia solutions are designed to make it possible for employees to collaborate and work more effectively. The results are a more collaborative environment, increased worker productivity and the ability to be much more responsive to customer needs. Malcolm Collins
ability athletes attend community continue create economic excited explore forum girls global help models positive role serve social support throughout ways women
Women athletes have the ability to serve as role models for women and girls throughout the world. I am excited to attend the World Economic Forum to explore ways in which the global community can continue to support athletes? ability to help create positive social change. Monica Seles
ability footwear market retain rights
We retain the footwear rights and the ability to market our athletes. Paul Harrington
access bush business considers creators cut decision disgrace jobs main outrageous owners president repeatedly small
It is a disgrace that while President Bush has repeatedly told small business owners that he considers them the main creators of jobs in America, his administration is making such an outrageous decision that will cut off their access to capital. Terry McAuliffe
access centers collection currently customers deadline guards hope immediate materials means problems provide trained understand until using written
This means that residents currently using the collection centers without a decal should not have any problems with access until May 1. All of our collection center guards have been trained on the new decal policy, and they have written materials to provide residents, so we hope that center customers will understand that they are not under an immediate deadline to get their decal. Robert Holden
accessible across boundaries costs draw equipment kids needy start
We want the kids to show up. We want to be accessible for any needy kid. It costs $300-400 to get started because the equipment is so expensive. We want to draw kids across boundaries to start relationships. Greg Porter
access basic economic education element social
A basic element of the American dream is equal access to education as the lubricant of social and economic mobility. Nicholas Kristof
children education ensure enter gradually great high indiana integrated prepared quality schools shame state system
Pre-K is gradually being integrated into the K-12 education system nationwide. In a state that has so many great universities like Purdue, Indiana and Notre Dame, it's a shame (Indiana) won't do more to ensure its own children are better prepared to enter those schools with high quality pre-K. Libby Doggett
children created happy parents
Children aren't happy with nothing to ignore, and that's what parents were created for. Ogden Nash
children years childhood
When I was seven years old, I fell in love with a series published by Bobbs-Merrill called 'The Childhood of Famous Americans.' In it, historical figures like Clara Barton, Nancy Hanks, Elias Howe, Patrick Henry, and dozens more came to life for me as children. Ann Hood
children night want
I guess I so desperately want to see us put this planet right. It's so horrifying to me that a fifth of us are starving every night, and that forty thousand children die every single day. Ann Druyan
children expectations way
Children have an uncanny way of living up - or down - to what is expected of them. Ann Landers
children halloween years
In recent years, there have been reports of people with twisted minds putting razor blades and poison in taffy apples and Halloween candy. It is no longer safe to let your child eat treats that come from strangers. Ann Landers
children names done
If you have a good name, if you are right more often than you are wrong, if your children respect you, if your grandchildren are glad to see you, if your friends can count on you and you can count on them in time of trouble, if you can face your God and say "I have done my best," then you are a success. Ann Landers
children parent inheritance
Parents do not owe their progeny an inheritance no matter how much money they have. One of the surest ways to produce loafers and freeloaders is to let children know that their future is assured. Ann Landers
children successful finals
What the majority of American children needs is to stop being pampered, stop being indulged, stop being chauffeured, stop being catered to. In the final analysis, it is not what you do for you children but what you have taught them to do for themselves that will make them successful human beings. Ann Landers
decide enjoy faster freedom game hard less liked matter sure time tv wants
said Simms. ''It's a matter of time. The game is much faster in the TV booth. It's so hard to get it done in a three-man booth. There's less time to talk. With only two guys, Troy will be able to set it up, and decide what he wants to show and tell. I'm sure he's going to enjoy it. I'm also sure he liked Cris and working with him. But this is about freedom. Dick Enberg
decided direction kid strong
We decided to go in another direction at quarterback. He's (Leak) a big, strong kid and he's got a big-time arm. Jason Gibson
decided developer eminent forced heard hill homes proposal selected taken
We don't think the residents of Cramer Hill should be forced out of their homes and that their homes should be taken by eminent domain. They had already selected the developer and decided on the proposal they would use before they had objectively evaluated the neighborhood and heard from the public. Olga Pomar
decided happened last oxford since step thursday
Since it happened in our area, and it?s the Oxford Lions, we thought we?d step up. We decided last Thursday at our meeting. We wanted to get it out and do it quick. Nancy Allen
decide develop health less local poor risking stay
Shelters here are less than comfortable, and poor sanitation can develop quickly. Residents who decide to stay in the county, even those who go to local shelters, are risking their health and safety. Brian Adams
decided emotional good guess hard
I thought about it long and hard and decided that's it. It's a very emotional moment, but I guess every good thing has to come to an end. Sanath Jayasuriya
decided open people process understand vitally
Transparency is vitally important in what can be a very opaque process in Brussels. We've decided to open this up so people can understand the issues. Horacio Gutierrez
decision feelings brain
When I realized I could actually make my decisions, it was a very strange feeling. It's like a switch went off in my brain, like, "Oh, then why am I doing this? I don't enjoy this, so I'm just gonna stop doing this." Taylor Momsen
decision age quitting
Once I got to an age where I was old enough to make my own decisions, I quit everything and did what I actually wanted to do, which was start a band. Taylor Momsen
decision able management
When I'm in management meetings when we're deciding my future, those decisions are left up to me. I'm the one who has to go out and fulfill all these obligations, so I should be able to choose which ones I do or not. That's the part of my life where I feel most in control. Taylor Swift
decision cowardice
The thing that cowardice fears most is decision Soren Kierkegaard
decision television killers
You don't wanna know the sinner. You don't wanna know the killer. Because it's you. Television is stalling evolution. Medication is stalling evolution. Evolution is stalling revolution. Evolution, revolution. Collaboration, the start of revolution. My decision, the start of revolution. Revolution, the start of evolution. Revolution, evolution. Tablo
decision coaches ifs
You can't really make the decision for the coaches. I don't know what's going to happen. If I knew, then I would tell you, but I don't. Ron Dayne
decision limits made
I just get really defensive as soon as anyone comes near my personal life. I made a decision early on that it's strictly off-limits. No exceptions. Samantha Morton
decision political pressure
...experience should warn us that tough and unpopular decisions are only made under intense political pressure produced by urgent necessity. Tom McClintock
decision firsts second-best
I'd rather have a second-best decision diligently pursued than a first-best decision lackadaisically pursued. Tom Landry
expect growth half profit second
We expect profit growth to decelerate in the second half of 2004. Abby Cohen
expect market number positive response sort strong
I would expect a strong payrolls number Friday, and for the market to have some sort of positive response to it, even if it's just very short-term. Robert Long
expect girls guys missing several various
We were missing several girls (for various reasons), and all of them would've placed. I really did expect the guys to win. Todd Barker
expectation fact higher life lower pay public service
It is a fact of life that the higher you go in public service and the higher your pay is the lower the expectation of privacy. Thomas Burke
expect february hear january known nicely quarter sales since
We expect to hear that Harley-Davidson sales were up nicely during the quarter since we had known that January and February sales were up 10%. Robin Farley
expect good seattle super
I thought Seattle was a very good team, but did I expect them to be in the Super Bowl? I thought if they were going to be there they'd have to go through us. Michael Strahan
expect half hit level market sometime
I think we will hit the $600 level sometime this year. I don't know if we will make it during the first half of the year, but I expect to see that level by year-end. The market is that strong. Joe Foster
expect strong trend
I think this is just a hiccup in a very strong trend that we expect to continue. Anthony Crescenzi
expect people state
I think we can go to state and do really well. A lot of people don't expect us to do anything. Matt Brown
naturally sports
I think I'm skilful enough, but I don't consider myself a naturally talented, gifted sports person. Tony McCoy
perfect trying kind
I'm far from perfect but I have managed to earn a good deal of respect by trying to implement the kinds of things you learn about. Mark Martin
perfect imperfection inward
... when one's outward lot is perfect, the sense of inward imperfection is the more pressing. George Eliot
perfect forbidden-love relation
Perfect love has a breath of poetry which can exalt the relations of the least-instructed human beings. George Eliot
perfect results motive
Pure motives do not insure perfect results. Christian Nestell Bovee
perfect needs flaws
You are perfect exactly as you are. With all your flaws and problems, there's no need to change anything. All you need to change is the thought that you aren't good enough. J. Cole
perfect actors tone
I loved Peter Sellers. I thought he was the perfect mix of physical comedy with out-of-the-box humor. I loved his tone; I loved his physicality; I loved everything about what he was doing as a comedic actor. J. B. Smoove
perfect imperfection boring
Being perfect is boring. It's the imperfections that make us perfect. Jessie J
perfect people skins
People can perfect whatever facade they want, but everyone holds their sins close to their skin. Jeaniene Frost
perfect mind want
There's kind of a Zen aspect to bowling. The pins are either staying up or down before you even throw your arm back. It's kind of a mind-set. You want to be in this perfect mind-set before you released the ball. Jeff Bridges
respect soldier three
There exist only three beings worthy of respect: the priest, the soldier, the poet. To know, to kill, to create. Charles Baudelaire