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ability again along artificial chain copies critical cut designing easily engineer genes millions paste pieces process putting rapidly sites specific spots together using
To make use of artificial genes we need to make millions of copies of them for experiments using a process called polymerase chain reaction. By putting restriction sites into specific spots along the gene, we can cut it into bite-sized pieces that are easily duplicated millions of times. So the ability to cut and paste genes back together again is critical for designing genes to the right specifications, rapidly replicating them and putting them into vectors to genetically engineer cells. Jef Boeke
ability again along artificial chain copies critical cut designing easily engineer genes millions paste pieces process putting rapidly sites specific spots together using
To make use of artificial genes, we need to make millions of copies of them for experiments using a process called polymerase chain reaction. By putting restriction sites into specific spots along the gene, we can cut it into bite-sized pieces that are easily duplicated millions of times. So the ability to cut and paste genes back together again is critical for designing genes to the right specifications, rapidly replicating them and putting them into vectors to genetically engineer cells. Jef Boeke
ability came everybody football plays terrific
Everybody got their money's worth. It was a terrific football game. It came down to our ability to make some plays in the end. Marty Schottenheimer
ability applaud aspects children concerned customers demand exploit helping hopefully horrific increases pimps pioneering sexual sexually terrible toward ways women
Everyone who is concerned about the sexual victimization of children and women should applaud the enactment of this legislation. It significantly increases the ability of authorities to go after the pimps and the customers who exploit these young women and children sexually and in such horrific ways. The so-called End Demand aspects of this legislation are pioneering in many ways and hopefully will go a long way toward helping to end the terrible sexual exploitation going on in our nation. Richard Land
ability based cognitive form mental specific
Based on her cognitive deficits, yes, she lacked the mental capacity, the cognitive ability to form the specific intent, Jonathan Crisp
ability accelerate beginning consumers continue economic false funk hopes pace raising relative remain tend
At the end of a long economic expansion, consumers tend to be overconfident relative to their spending; raising false hopes about the ability of consumers to continue spending. At the beginning of a recovery, consumers remain in a funk even as they accelerate their pace of spending. Carl Steidtmann
ability american-artist animated business draw guys living mostly newspaper people
At that time, the people that were in the animated film business were mostly guys who were unsuccessful newspaper cartoonists. In other words, their ability to draw living things was practically nil. Marc Davis
ability communication customer designed drive employee employees focus increase increased multimedia possible providing responsive results solutions work worker
At Nortel, our focus is providing multimedia communication capabilities that increase employee productivity and drive innovation, ... Our new IP multimedia solutions are designed to make it possible for employees to collaborate and work more effectively. The results are a more collaborative environment, increased worker productivity and the ability to be much more responsive to customer needs. Malcolm Collins
ability athletes attend community continue create economic excited explore forum girls global help models positive role serve social support throughout ways women
Women athletes have the ability to serve as role models for women and girls throughout the world. I am excited to attend the World Economic Forum to explore ways in which the global community can continue to support athletes? ability to help create positive social change. Monica Seles
desire intense stage
You feel the intense desire to do good, to do right thing. At this stage, you also feel helpless. Chin-Ning Chu
good horse might people rabbit rabbits running using
Sometimes running a rabbit works, sometimes it backfires. People have been using rabbits for hundreds of years. The rabbit has to be a good horse because they might not necessarily be able to do the job. Nick Zito
good
I've run into some S.O.B. directors, but I gave them back as good as I got. Carroll O'Connor
good huge run third win
It was a huge win for us. We got on a good run in the third quarter. Jody Bass
good great leaders-and-leadership seniors students
These seniors were great leaders. They were all good students and great people. Lynn Meger
good maintain news property trying
Is it a challenge, but the good news for them is that it has been a sleepier brand, so it's not as if they are trying to maintain a property at its peak. Marshal Cohen
good meeting nobody team
I showed up at the team meeting (Saturday) and nobody told me to get out, so it's good news, Craig Krenzel
good imagine job reason till
I see no reason to quit. I can't imagine walking away from being Big Bird. I mean, that's an awfully good job that there's not too many of them. So I just want to keep doing it till I can't do it anymore. Caroll Spinney
good happy
I should be happy but right now it doesn't feel so great. It's a good accomplishment but I know it could have better. Matt Lee
good
I should be European, man. I'm long and lean. I'd look good in a trench coat. J. B. Smoove
helped points school smallest teams ten wrestlers
Ten of our 14 wrestlers scored points which helped us today. There were 15 teams and we didn't know what to expect. We were the smallest school there. Kirby Moses
help sure
We didn't play bad, ... But we sure didn't do a lot to help ourselves. Deon Grant
helps obviously possession time
Time of possession obviously helps us. We've just got to keep on working. Derek Smith
leadership basketball practice
I just love the game of basketball so much. The game! I don't need the 18,000 people screaming and all the peripheral things. To me, the most enjoyable part is the practice and preparation. Bobby Knight
leadership business crazy
When trouble arises and things look bad, there is always one individual who perceives a solution and is willing to take command. Very often, that individual is crazy. Dave Barry
leadership steps arrogant
God will never direct us to be prideful, arrogant and unforgiving, immoral or slothful or full of fear. We step into these things because we are insensitive to the leadership of the Holy Spirit within us. Charles Stanley
leadership decision benefits
Leadership requires the courage to make decisions that will benefit the next generation. Alan Autry
leadership sports winning
So what if I never win my fifth gold medal; It's only one end of the string. It's competing that matters. It's proving that there is a place for guys like me in sports. It's a persona challenge to extend myself. Al Oerter
leadership basketball sports
Winning is overrated. The only time it is really important is in surgery and war. Al McGuire
leadership sports zero
I believe in a business boarding up early. If you make a mistake, you put the boards in the window of the store and say, "Hey, I made a mistake." Let me take two shots in the arm and a punch on the nose and let me get on to the next thing. I don't believe in worrying over failures. I worry about successes. This is opposite from most people. Most people zero in on their failures. I try to keep all my attention on a pyramid type philosophy rather than the averaging-down philosophy. Al McGuire
leadership voice ideals
The ideal leader is one who hears the voice of God, and beckons on as the voice calls him and them. Aiden Wilson Tozer
leadership work reality
I would rather die than be in the United States Senate. I would be bored to death. Could you imagine me, banging around that chamber with 99 other people, asking for a motion on the amendment in the subcommittee? Forget it...You'd watch me just walk out and walk right into the Potomac River and drown. That would be it. Chris Christie
teacher sex children
People don't want children to know what they need to know. They want their kids to know what they ought to need to know. If you're a teacher you're in a constant battle with mildly deluded adults who think the world will get better if you imagine it is better. You want to teach about sex? Fine, but only when they're old enough to do it. You want to talk politics? Sure, but nothing modern. Religion? So long as you don't actually think about it. Otherwise some furious mob will come to your house and burn you for a witch. Nick Harkaway
teacher writing class
The teacher always used me as an example to the class of good English and good storytelling because we all had to write the same stories. But she used to make me go out front - which I hated - and read my story to the class and I would get huge applause. Not because of who I was but because they truly enjoyed the stories I wrote. Michael Jackson
teach
I have come not to teach but to awaken. Meher Baba
teacher lessons forgotten
Fear is not a good teacher. The lessons of fear are quickly forgotten. Mary Catherine Bateson
teacher jesus plato
John Adams was a farmer, Abraham Lincoln a small town lawyer. Plato and Socrates were teachers. Jesus was a carpenter. To equate wisdom and judgement with occupation is at best insulting. Mark Sheppard
teacher thinking trying
Now I realize that I have to let everyone take what they have to take from the film. No matter what I think about the film, it becomes a little irrelevant. I think I would say that the film is trying to show us that - and I spoke about that earlier - we have to let the teachers invest in their own classroom. There's no use in trying to control everything. Education is fundamental. Philippe Falardeau
teacher real bad-friend
We get through life and this is part of the education process also. In real life, we meet bad bosses and good bosses and good friends and bad friends. I think we should let the teachers do their work and not impose too much stuff on them. Philippe Falardeau
teacher jesus father
I believe in God - not in a Catholic God; there is no Catholic God. There is God, and I believe in Jesus Christ, his incarnation. Jesus is my teacher and my pastor, but God, the Father, Abba, is the light and the Creator. This is my Being. Pope Francis
teacher children joy
A good teacher does not get lost in the details, but points to what is essential so that the child or student can find meaning and joy in life. Pope Francis
team winning texas
During my 40-year coaching career at West Point, Indiana and Texas Tech, my teams reached the Final Four on five occasions, winning the national championship three times. Bobby Knight
team successful leader
To be successful in coaching you have to treat your team like a family. The leader needs backing from everyone. Morgan Wootten
team ties houston
I still have very close ties to Houston and the Astros because that was my first team. Joe Morgan
team games effort
I've figured out that life in general is a team effort; it's a team game. Joe Namath
team struggle kissing
I wanna kiss you. I couldn't care less about the team struggling. What we know is we can improve. Chad Pennington, our quarterback, missed the first part of the season, and we struggled. We're looking to next season. We're looking to make a noise now, and I wanna kiss you! Joe Namath
team winning six
When you have five or six potential hall of famers on your team ... that's when you win penants and championships. The only thing you have to be concerned about is getting the ballplayers to bed on time. Joe DiMaggio
team good-person persons
I'm a good person but a shitty writer. You're a shitty person but a good writer. We'd make a good team. John Green
team cocky hockey
Hopefully someone on their team addresses it, because, uh, I'm not saying I'm going to do it, but something might happen to him if he continues to be that cocky. Mike Richards
team careers impact
I tell people in their careers, 'Look for growth. Look for the teams that are growing quickly. Look for the companies that are doing well. Look for a place where you feel that you can have a lot of impact.' Sheryl Sandberg
understanding curiosity mind
The greatest weapons in the conquest of knowledge are an understanding mind and the inexorable curiosity that drives it on. Isaac Asimov
understanding levels fox-news
There are a lot of folks at Fox News who bring a certain level of sophistication to their understanding of politics. David Shuster