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ability again along artificial chain copies critical cut designing easily engineer genes millions paste pieces process putting rapidly sites specific spots together using
To make use of artificial genes we need to make millions of copies of them for experiments using a process called polymerase chain reaction. By putting restriction sites into specific spots along the gene, we can cut it into bite-sized pieces that are easily duplicated millions of times. So the ability to cut and paste genes back together again is critical for designing genes to the right specifications, rapidly replicating them and putting them into vectors to genetically engineer cells. Jef Boeke
ability again along artificial chain copies critical cut designing easily engineer genes millions paste pieces process putting rapidly sites specific spots together using
To make use of artificial genes, we need to make millions of copies of them for experiments using a process called polymerase chain reaction. By putting restriction sites into specific spots along the gene, we can cut it into bite-sized pieces that are easily duplicated millions of times. So the ability to cut and paste genes back together again is critical for designing genes to the right specifications, rapidly replicating them and putting them into vectors to genetically engineer cells. Jef Boeke
ability came everybody football plays terrific
Everybody got their money's worth. It was a terrific football game. It came down to our ability to make some plays in the end. Marty Schottenheimer
ability based cognitive form mental specific
Based on her cognitive deficits, yes, she lacked the mental capacity, the cognitive ability to form the specific intent, Jonathan Crisp
ability accelerate beginning consumers continue economic false funk hopes pace raising relative remain tend
At the end of a long economic expansion, consumers tend to be overconfident relative to their spending; raising false hopes about the ability of consumers to continue spending. At the beginning of a recovery, consumers remain in a funk even as they accelerate their pace of spending. Carl Steidtmann
ability american-artist animated business draw guys living mostly newspaper people
At that time, the people that were in the animated film business were mostly guys who were unsuccessful newspaper cartoonists. In other words, their ability to draw living things was practically nil. Marc Davis
ability communication customer designed drive employee employees focus increase increased multimedia possible providing responsive results solutions work worker
At Nortel, our focus is providing multimedia communication capabilities that increase employee productivity and drive innovation, ... Our new IP multimedia solutions are designed to make it possible for employees to collaborate and work more effectively. The results are a more collaborative environment, increased worker productivity and the ability to be much more responsive to customer needs. Malcolm Collins
ability athletes attend community continue create economic excited explore forum girls global help models positive role serve social support throughout ways women
Women athletes have the ability to serve as role models for women and girls throughout the world. I am excited to attend the World Economic Forum to explore ways in which the global community can continue to support athletes? ability to help create positive social change. Monica Seles
ability footwear market retain rights
We retain the footwear rights and the ability to market our athletes. Paul Harrington
artificial cake design extreme forces good reality
I think the good thing about Dogme is that it forces you into an extreme sense of reality because there's no artificial light and no set design and all of those icings on the cake that you usually have on a movie. Susanne Bier
damage hear positive process rebuilding speeches
We hear speeches about how the rebuilding is in process and technically it's true, but those positive speeches overshadow just how little is being done and just how much damage there is here. Matt Farley
damaged unknown
Unknown to myself, I damaged all my joints. Barry Gibb
fall self fall-into-place
When you become confident with your inner-self, the outside's just going to fall into place. Billy Blanks
fall shoes half
I had these shoes made and 2 to 3 inch lifts inside and the heel was another 2 and half inches. I walked around that way, wherever I could without falling over. Bobby Darin
fall rising wave
All motion in this universe is in the form of waves, successively rising and falling. Swami Vivekananda
fall keys order
But the world is ever more interdependent. Stock markets and economies rise and fall together. Confidence is the key to prosperity. Insecurity spreads like contagion. So people crave stability and order. Tony Blair
fall people granted
You get into your late fifties, people start falling like flies all around you. I don't take life for granted any more. I'm really glad to be here. Tom Petty
fall men age
Old age is better for women than for men. First of all, they have less far to fall, since their lives are more mediocre than those of most men. Simone de Beauvoir
fall dust one-day
She would never change, but one day at the touch of a fingertip she would fall to dust. Simone de Beauvoir
fall thinking doe
It seemed to me certain, and I still think so today, that one can never wrestle enough with God if one does so out of pure regard for the truth. Christ likes us to prefer truth to him because, before being Christ, he is truth. If one turns aside from him to go toward the truth, one will not go far before falling into his arms. Simone Weil
fall holiday thinking
I realized that if I went snowboarding, you can't think of anything else when you're snowboarding. You can't hesitate or think about anything other than not falling off and breaking your neck. If you want a holiday where you're not gonna think about work and you're not gonna think about anything, snowboarding is the best way to do it. Or skiing, I guess. I don't ski, so I don't know. Simon Pegg
games good honestly honor miss remember
It was an honor to be a part of history, but honestly I don't remember all the games we've played. I do remember the trips, though, and that's what we will miss the most ? all those good times. Michael Beasley
game minute next seven
Turnovers were the killers tonight. One minute, it's a one-point game, the next minute it's seven points. Matt Copeland
game home points receiver score team throw watching
We don't score 30 points a game. We don't have a receiver who can go and get you 180 yards. We don't have a quarterback who's going to throw for 350. But I'll tell you what we do have: A team that's going to the AFC Championship Game. All them big-time offenses, you know where they're at? Home watching us. Shannon Sharpe
game run suit tail
We don't run the game for betting. To have it regulated to suit punters would see the tail wagging the dog. David Gallop
game green james katrina needed plays
We don't really have any stars. But we do have some depth. Jasmine Green and Katrina James went into the game and made some big plays for us when we needed them. Matt Koff
game happen hope
You hope that every game is not close, but in this game it doesn't happen that way. The disappointing thing is when we give up two restart goals. Neil Roberts
game guys lead loss maybe quarter sooner third understand won
You hope, sooner or later, the young guys understand and get it. When you have a lead like that, you can't come out in the third quarter and think the game is over. Maybe a loss like this, when we know we should have won the game, that will do it. Byron Scott
game hear maybe people worried
You hear that a lot. Maybe that's just the way people see it. But you can't really be worried about that. You've just got to get out there and play the game of basketball. Marcus Ginyard
game instinct nerves plays
To have those nerves to make those plays at the end of a game isn't something you really can be taught. It's instinct I think. Chauncey Billups
leadership basketball practice
I just love the game of basketball so much. The game! I don't need the 18,000 people screaming and all the peripheral things. To me, the most enjoyable part is the practice and preparation. Bobby Knight
leading
We were leading 7-2 in the game. We just didn't get it done. Bobby Howard
leader boss unions
My problem with public sector union leaders, the bosses, has been they stood in the way of protecting the taxpayer. Scott Walker
leadership business crazy
When trouble arises and things look bad, there is always one individual who perceives a solution and is willing to take command. Very often, that individual is crazy. Dave Barry
leadership steps arrogant
God will never direct us to be prideful, arrogant and unforgiving, immoral or slothful or full of fear. We step into these things because we are insensitive to the leadership of the Holy Spirit within us. Charles Stanley
leadership decision benefits
Leadership requires the courage to make decisions that will benefit the next generation. Alan Autry
leadership sports winning
So what if I never win my fifth gold medal; It's only one end of the string. It's competing that matters. It's proving that there is a place for guys like me in sports. It's a persona challenge to extend myself. Al Oerter
leadership basketball sports
Winning is overrated. The only time it is really important is in surgery and war. Al McGuire
leadership sports zero
I believe in a business boarding up early. If you make a mistake, you put the boards in the window of the store and say, "Hey, I made a mistake." Let me take two shots in the arm and a punch on the nose and let me get on to the next thing. I don't believe in worrying over failures. I worry about successes. This is opposite from most people. Most people zero in on their failures. I try to keep all my attention on a pyramid type philosophy rather than the averaging-down philosophy. Al McGuire
market sometime
The new Z4s are not in the market yet. It will be sometime this spring. Bobby Hitt
rule shower
That was two too many. The first rule was you must shower every day. Marty Smith
tv-shows feet wife
I'd love to have my own TV show, in the way that Julianna Margulies has 'The Good Wife,' or a lovely ensemble show, like 'Six Feet Under. Lara Pulver
tv-shows people tvs
I spend 80% of my time in my restaurants. Taping my TV shows doesn't take much time, and then they get aired a lot. That's the thing people don't realize. Bobby Flay
tvs shows
You don't have to own a TV network to go out and do a cool show. Bill Gates