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ability again along artificial chain copies critical cut designing easily engineer genes millions paste pieces process putting rapidly sites specific spots together using
To make use of artificial genes we need to make millions of copies of them for experiments using a process called polymerase chain reaction. By putting restriction sites into specific spots along the gene, we can cut it into bite-sized pieces that are easily duplicated millions of times. So the ability to cut and paste genes back together again is critical for designing genes to the right specifications, rapidly replicating them and putting them into vectors to genetically engineer cells. Jef Boeke
ability again along artificial chain copies critical cut designing easily engineer genes millions paste pieces process putting rapidly sites specific spots together using
To make use of artificial genes, we need to make millions of copies of them for experiments using a process called polymerase chain reaction. By putting restriction sites into specific spots along the gene, we can cut it into bite-sized pieces that are easily duplicated millions of times. So the ability to cut and paste genes back together again is critical for designing genes to the right specifications, rapidly replicating them and putting them into vectors to genetically engineer cells. Jef Boeke
ability came everybody football plays terrific
Everybody got their money's worth. It was a terrific football game. It came down to our ability to make some plays in the end. Marty Schottenheimer
ability applaud aspects children concerned customers demand exploit helping hopefully horrific increases pimps pioneering sexual sexually terrible toward ways women
Everyone who is concerned about the sexual victimization of children and women should applaud the enactment of this legislation. It significantly increases the ability of authorities to go after the pimps and the customers who exploit these young women and children sexually and in such horrific ways. The so-called End Demand aspects of this legislation are pioneering in many ways and hopefully will go a long way toward helping to end the terrible sexual exploitation going on in our nation. Richard Land
ability based cognitive form mental specific
Based on her cognitive deficits, yes, she lacked the mental capacity, the cognitive ability to form the specific intent, Jonathan Crisp
ability accelerate beginning consumers continue economic false funk hopes pace raising relative remain tend
At the end of a long economic expansion, consumers tend to be overconfident relative to their spending; raising false hopes about the ability of consumers to continue spending. At the beginning of a recovery, consumers remain in a funk even as they accelerate their pace of spending. Carl Steidtmann
ability american-artist animated business draw guys living mostly newspaper people
At that time, the people that were in the animated film business were mostly guys who were unsuccessful newspaper cartoonists. In other words, their ability to draw living things was practically nil. Marc Davis
ability communication customer designed drive employee employees focus increase increased multimedia possible providing responsive results solutions work worker
At Nortel, our focus is providing multimedia communication capabilities that increase employee productivity and drive innovation, ... Our new IP multimedia solutions are designed to make it possible for employees to collaborate and work more effectively. The results are a more collaborative environment, increased worker productivity and the ability to be much more responsive to customer needs. Malcolm Collins
ability athletes attend community continue create economic excited explore forum girls global help models positive role serve social support throughout ways women
Women athletes have the ability to serve as role models for women and girls throughout the world. I am excited to attend the World Economic Forum to explore ways in which the global community can continue to support athletes? ability to help create positive social change. Monica Seles
giving people leader
Some citizens are so good that nothing a leader can do will make them better. Others are so incorrigible that nothing can be done to improve them. But the great bulk of the people go with the moral tide of the moment. The leader must help create that tide. Some coaches pray for wisdom. I pray for 260-pound tackles. They'll give me plenty of wisdom. Chuck Noll
giving who-we-are circumstances
But regardless of our circumstances, they do not define us - not unless we give in and let them. Circumstances never determine who we are; they reveal who we are. Chuck Pagano
giving judging feelings
Just as we descend into our consciences to judge of actions which our minds can not weigh, can we not also search in ourselves for the feeling which gives birth to forms of thought, always vague and cloudy? Alfred de Vigny
giving decision kind
I'm just kind of taking whatever life gives me and hoping that I make the right decision. Amy Smart
giving advice trying
I listen. I like to give advice. Mostly, Ill just try to listen to my friends, and theyll say the same thing over and over again. Amy Sedaris
giving answers teach
You can't say history teaches us this or that; it gives us more questions than answers, and many answers to every question. Amin Maalouf
giving environmental energy
If you ask me, it'd be a little short of disastrous for us to discover a source of clean, cheap, abundant energy because of what we would do with it. We ought to be looking for energy sources that are adequate for our needs, but that won't give us the excesses of concentrated energy with which we could do mischief to the earth or to each other. Amory Lovins
giving rose trying
Let us try to see things from their better side: You complain about seeing thorny rose bushes; Me, I rejoice and give thanks to the gods That thorns have roses. Alphonse Karr
giving perfect littles
It had been a wonderful evening and what I needed now, to give it the perfect ending, was a little of the Ludwig Van. Anthony Burgess
government firsts students
At first I intended to become a student of the Senate rules and I did learn much about them, but I soon found that the Senate hadbut one fixed rule, subject to exceptions of course, which was to the effect that the Senate would do anything it wanted to do whenever it wanted to do it. Calvin Coolidge
government surprise no-excuses
We will be a consultative, collegial government. No surprises, no excuses. That's what you'll get under the Coalition. Tony Abbott
government coalitions taxes
Taxes will always be lower under a Coalition government Tony Abbott
government reduction said
There is much to be said for an emissions trading scheme. It was, after all, the mechanism for emission reduction ultimately chosen by the Howard government. Tony Abbott
government quality determined
The quality of government, I suppose, is determined not by what happens, but by how you react to what happens. Tony Abbott
government people excuse
The Australian people expect the Government to govern, they don't expect it to make excuses. Tony Abbott
government priorities three
Ask me my three main priorities for Government, and I tell you: education, education, and education. Tony Blair
government challenges next
In government you carry each hope; each disillusion. And in politics it's always about the next challenge. Tony Blair
government priorities arrogant
It is not an arrogant government that chooses priorities, it's an irresponsible government that fails to choose. Tony Blair
healthy skins passionate
Aside from eating healthy and taking care of my body, I've always been passionate about skincare. It's an essential part of my daily regimen, which is why I decided to create my own skincare line, DEVONNE by Demi. When my skin is clean, I feel my best! Demi Lovato
healthy care firsts
Those obsessed with health are not healthy; the first requisite of good health is a certain calculated carelessness about oneself. Sydney J. Harris
health catching seems
Like everybody else, when I don't know what else to do, I seem to go in for catching colds. George Jean Nathan
health help issue pour
We have to pour some chlorine in there to help sanitize it a bit. It's still another health issue for my daughter. David Clark
healthy way-to-live looks
If success is really dependent on someone liking you or not liking you, and you have to teeter on that kind of tightrope of how you're supposed to act and how you're supposed to look and who you are, it's just not a healthy way to live. Ellen DeGeneres
health rural
Without Manatee Rural Health, we could not make this work. Brian Flynn
helped points school smallest teams ten wrestlers
Ten of our 14 wrestlers scored points which helped us today. There were 15 teams and we didn't know what to expect. We were the smallest school there. Kirby Moses
help sure
We didn't play bad, ... But we sure didn't do a lot to help ourselves. Deon Grant
helps obviously possession time
Time of possession obviously helps us. We've just got to keep on working. Derek Smith
jobs people vote
How many people disapprove of the job the Conservatives are doing? Seventy percent. Of those same people, how many will vote for them again? ...Seventy percent. What the f-k? Where did they take this poll, at an S&M parlor? Bill Hicks
jobs college class
Every single thing I`ve done, from the Affordable Care Act to pushing to raise the minimum wage, to making sure that young people are able to go to college and get good job training, to what we`re pushing now in terms of sick paid leave,everything I do has been focused on how do we make sure the middle class is getting a fair deal. Barack Obama
jobs kings opportunity
One of the things about Steve Jobs is that he gives us an opportunity to look at the disjuncture between that world and the world he claimed that Apple represented, the "Think different" world of Martin Luther King and Rosa Parks and Gandhi. Alex Gibney
jobs feel-better technology
Jobs would have ever have asserted that Bill Gates was not serious about technology. He was a huge pioneer in that world, albeit doing something quite different in approach from what Steve did. He was dismissive of Gates' foundation work as something he did to make himself feel better. Alex Gibney
jobs blessed people
I don't know why people say I'm not supposed to be doing what I'm doing. I'm just trying to do my job. I'm blessed, and I'm glad that I'm blessed. Albert Pujols
jobs games preparation
Preparation is very important. The pitcher is going to do his job and prepare for you so you as a hitter must do the same. I always watch videotape of pitchers before the game and even sometimes during. Albert Pujols
jobs loss class
The economic recession in America wasn't caused by bad luck; it was caused by bad Republican policies. But the Republican candidates are doubling down on the same flawed policies that led to the loss of 3.6 million jobs in the final months of 2008 and gravely affected middle class families across America. Debbie Wasserman Schultz
jobs believe cutting
[Donald] Trump and all the Republicans believe in the theory of trickle down economics which is a theory discredited even by the author himself David Stockton. The theory suggests that if we take care of the people at the top, if we cut taxes for the wealthy, if we make sure they are doing really well, then the investments that they make in the economy and the jobs that will create, will make everything grow and it will have a trickle down effect on the rest of us. Debbie Wasserman Schultz
jobs months administration
When we took over the economy, we were losing 800 thousand jobs a month under the [George W.] Bush administration. Debbie Wasserman Schultz
people time touch
This is the first time when we can touch people when they have been devastated. Tim Cox
people standing union
This is not a union that is struggling. People see we are standing up for what they want and they want to join. Mark Serwotka
people triumph
This is not a triumph for Chirac. People didn't go to vote. Dominique Moisi
people problem turning
This is not about turning people away from the park, ... The problem is not that there's too many people. The problem is there's too many cars. Bruce Babbitt
people
This is not to make it easier, it's just to entice people to come. Julie Taymor
people recession report represents simply
This is simply what a recession is like. This report represents real people and real pain, especially around the holidays. Bill Cheney
people
This is really good. People are being vaccinated. Barbara Schneeman
people season surprising
This is probably the most surprising season, my season and the team's. For a lot of people around the league. Jaromir Jagr
people preserve wonderful
This is so wonderful to see all of these people who are working to preserve our history, Harry Price
responsibility men doe
I fought for the values of responsibility, and I'm not a man who does not accept his responsibilities. Nicolas Sarkozy
responsibility acting talent
Talent is an accident of genes - and a responsibility. Alan Rickman
responsibility character scripts
Responsibilities are relative. My responsibility is to a character in a script, to a part I'm playing. Al Pacino
responsibility primaries
My primary responsibility is to be funny, Al Madrigal
responsibility
A baby is a loud noise at one end and no sense of responsibility at the other. Ronald Knox