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ability again along artificial chain copies critical cut designing easily engineer genes millions paste pieces process putting rapidly sites specific spots together using
To make use of artificial genes we need to make millions of copies of them for experiments using a process called polymerase chain reaction. By putting restriction sites into specific spots along the gene, we can cut it into bite-sized pieces that are easily duplicated millions of times. So the ability to cut and paste genes back together again is critical for designing genes to the right specifications, rapidly replicating them and putting them into vectors to genetically engineer cells. Jef Boeke
ability again along artificial chain copies critical cut designing easily engineer genes millions paste pieces process putting rapidly sites specific spots together using
To make use of artificial genes, we need to make millions of copies of them for experiments using a process called polymerase chain reaction. By putting restriction sites into specific spots along the gene, we can cut it into bite-sized pieces that are easily duplicated millions of times. So the ability to cut and paste genes back together again is critical for designing genes to the right specifications, rapidly replicating them and putting them into vectors to genetically engineer cells. Jef Boeke
ability came everybody football plays terrific
Everybody got their money's worth. It was a terrific football game. It came down to our ability to make some plays in the end. Marty Schottenheimer
ability applaud aspects children concerned customers demand exploit helping hopefully horrific increases pimps pioneering sexual sexually terrible toward ways women
Everyone who is concerned about the sexual victimization of children and women should applaud the enactment of this legislation. It significantly increases the ability of authorities to go after the pimps and the customers who exploit these young women and children sexually and in such horrific ways. The so-called End Demand aspects of this legislation are pioneering in many ways and hopefully will go a long way toward helping to end the terrible sexual exploitation going on in our nation. Richard Land
ability based cognitive form mental specific
Based on her cognitive deficits, yes, she lacked the mental capacity, the cognitive ability to form the specific intent, Jonathan Crisp
ability accelerate beginning consumers continue economic false funk hopes pace raising relative remain tend
At the end of a long economic expansion, consumers tend to be overconfident relative to their spending; raising false hopes about the ability of consumers to continue spending. At the beginning of a recovery, consumers remain in a funk even as they accelerate their pace of spending. Carl Steidtmann
ability american-artist animated business draw guys living mostly newspaper people
At that time, the people that were in the animated film business were mostly guys who were unsuccessful newspaper cartoonists. In other words, their ability to draw living things was practically nil. Marc Davis
ability communication customer designed drive employee employees focus increase increased multimedia possible providing responsive results solutions work worker
At Nortel, our focus is providing multimedia communication capabilities that increase employee productivity and drive innovation, ... Our new IP multimedia solutions are designed to make it possible for employees to collaborate and work more effectively. The results are a more collaborative environment, increased worker productivity and the ability to be much more responsive to customer needs. Malcolm Collins
ability athletes attend community continue create economic excited explore forum girls global help models positive role serve social support throughout ways women
Women athletes have the ability to serve as role models for women and girls throughout the world. I am excited to attend the World Economic Forum to explore ways in which the global community can continue to support athletes? ability to help create positive social change. Monica Seles
allowed bit discovered freedom indians large later quite rocks though walk
Even though I was their captive, the Indians allowed me quite a bit of freedom. I could walk freely, make my own meals, and even hurl large rocks at their heads. It was only later that I discovered that they were not Indians at all but only dirty-clothes hampers. ![]()
allow appreciate came coaching enjoyed hard hard-work job past players program three work
I enjoyed coaching at Lamar the past three years and appreciate the hard work the players put in to making the program better. This will allow me to get back to doing the job I came back to Lamar to do in the first place. Billy Tubbs
allow chance days gives large lost maryland pool reason second state team teams top unlimited win
Go back into the days when Maryland lost the ACC championship to N.C. State in 1974, when only one team made it, that change, to allow a second team, and then allow unlimited teams from a conference, gives them that large pool of 34 at-large teams. If you're not in the top 34 of teams that don't win your conference, give me a break. You've had the chance to win games. You have no reason to complain. Jim Johnson
allow bench clear effort players pleased quality
I was pleased with out effort tonight. I was able to clear the bench and allow some players quality play time. Brad Smith
allow attempt department grew line might political public served
I grew up in the Justice Department. I served 12 years as a line lawyer in the public integrity section. This department under me will not have any kind of political interference. I will not allow political interference in the Justice Department. Those who might attempt to do that will be rebuffed. Eric Holder
allow area district enforce historic laws obey unfair
If we don't enforce laws of the historic district and allow things to slide, then it's unfair to homeowners that live in the area and do obey the rules. We don't have anything to defend. We are just doing our job. Jacqueline Salame
allow ask fully fund
If we don't ask for something that would allow us to be fully funded, I don't want the commissioners to not be able to fully fund us. Mike Kelley
allow bombing clear consider evidence meeting orderly pause president seemed
If there were to be clear and unambiguous evidence that (Yugoslav President Slobodan) Milosevic was meeting the conditions, and that he seemed to be doing so, then we would consider a bombing pause to allow an orderly withdrawal of his forces, George Robertson
allow cared conditions deeply family forcible sides spoke transfer various
She spoke on various sides of the issue, ... She spoke about how deeply she cared for the child, how much she did not want there to be a forcible transfer, but she also spoke about how she would never part with him, about how the family would never allow the transfer to take place under the conditions that were required. There were many contradictory statements. Doris Meissner
athletic conference finished games happy kids ninth overall players program rewarded second thrilled tied toughest western won
I'm very happy that our players have been rewarded for what they've accomplished this season. We won 20 games overall and finished tied for second in the Western Athletic Conference, which is the ninth toughest conference in the country. I'm thrilled that our program and these kids have been rewarded. Keith Richard
athletic ball hurt proud spoken state straight sure wake win won
I'm sure it didn't hurt because that was what they had spoken about. We weren't doing it to wake them up. We just wanted to win a ball game, and we're very proud that we won two straight (Mountain State Athletic Conference) titles. Rick Greene
athletic board campus chair chairs change decided good people periods seemed serve three university
In consultation with the University Committee, we decided that people should not serve as chair of the Athletic Board for inordinately long periods of time. Many departments on campus change chairs every two years or three years. It seemed like a good thing to do. John Wiley
athletic both feet good move physical situations struggled taller team
In both situations we struggled a little bit, but this team is a little more physical and a little taller than I think they're listed. They have good athletic ability, and they move their feet well. Randy Yoder
athletic baptism both fill fire goal hard humble injuries leadership looked mature minutes players scoring senior share
Injuries are part of the athletic experience. Many young players will be looked to to share these senior's minutes and fill the goal scoring void. Yet senior leadership is very hard to replicate. Baptism by fire will both humble and mature our young squad. Lori Walker
athletic comments director good help hope offended people president prove saying together
I'm not as offended as the people in my administration should be offended. I think we've got something good going. I think this can help us. The administration and I can come together and prove the comments he's saying are not true. I would hope our president and vice-president and athletic director would be offended by it. Dereck Whittenburg
athletic challenge definitely fast loves quick remember run team
I know they're an athletic and really quick team that loves to run the fast break. I remember Natasha, and she's just awesome. She does everything so well. She and Adrianne definitely will be a challenge to defend. Abbie Letz
athletic talented team youthful
I know we're a much more talented and athletic team than we've ever been. I also know that we're a much more inexperienced and youthful team than we've ever been in the past. Randy Edsall
athletic door five four front great john revolving
I know that while John was here he went through four or five athletic directors. It was like a revolving door and I don't know if he got a lot of support. But I have to look at what's in front of me. John is a great guy. David Coates
block building good
It's a good building block to get these two really good young players. Jim Rutherford
block air three
On the air, it's as if somebody draws a heavy drape to block any bias. I don't even feel it inside. It's the same curtain that keeps you from swearing. I've known broadcasters who can't get one sentence out in normal conversation without being profane, and yet, they can go on the air and talk for three hours and never slip once. Dick Enberg
calling hope last people positive somewhere
We hope to have at least 50 kids, somewhere between 50 and 75. The people calling are very excited, very positive about what we did last year. Thad Schultz
call certainly folks inclined interested reaching taken whatever
Certainly political capital-slash-celebrity attention, whatever you want to call it, certainly is part of the reason why I've been reaching out to CEOs. There's a lot of folks who probably would have taken a call from me before but are even more inclined now and are interested in what we're doing because of all the attention. Scott Walker
call chance customer particular profit
So long as there is a chance that a customer will call you with a problem, you don't really know what the profit is on that particular machine. Paul Nunes
call guy hate hurt people somebody speech
I hate it when people say somebody has a ""speech impediment"", even if he does, because it could hurt his feelings. So instead, I call it a ""speech improvement"", and I go up to the guy and say, ""Hey, Bob, I like your speech improvement."" I think this makes him feel better. ![]()
call cut desires difficult free leave leaving people pleasures strong wise
That fetter wise people call strong which drags down, yields, but is difficult to undo; after having cut this at last, people leave the world, free from cares, and leaving desires and pleasures behind. Friedrich Muller
call john people
Julian would call and Yoko or one of her people would say John was sleeping, Cynthia Lennon
call gonna honest lose women
You just need to be honest with how you're feeling. But, a lot of women are afraid of it because they think, 'Oh, they are going to take my baby away. They're gonna call me incompetent. I'm going to lose my job. I've got to be tough, it's a man's world.' Marie Osmond
call convey impression might opened prohibit seemed store testing trampoline tramps trying unless
If I ever opened a trampoline store, I don't think I'd call it Trampo-Land, because you might think it was a store for tramps, which is not the impression we are trying to convey with our store. On the other hand, we would not prohibit tramps from browsing, or testing the trampolines, unless a tramp's gyrations seemed to be getting out of control. ![]()
call crazy possibilities stay thinking trade until
I thought about it every day. It got crazy at the trade deadline, thinking of all the possibilities where I could have gone. But nothing's done until they call you and say, 'You're traded.' I feel like I'm here, and as of now, I'm going to stay until they make a decision. Lastings Milledge
guy singers heard
You ever heard of a guy named Jeff Buckley? He's one of the best singers I've ever heard. Sebastian Bach
guy rehearsal would-be
I'm not a guy that loves a lot of rehearsal, but it depends. It depends what it would be. Scott Speedman
guy lasts bad-times
Bad times don't last, bad guys do. Scott Hall
guy today tongue
In Hollywood today, it's cool for guys to wear nail polish and earrings in their lips and tongues. I don't get it. Scott Caan
guy filmmaker great-film
Working with David Ayer...the guy is a great filmmaker. Scott Eastwood
guy care needs
Care. Care more than you need to, more often than expected, more completely than the other guy. Seth Godin
guy
I love confidence in a guy. I don't have it, but there's nothing sexier. Todd Phillips
guy matter band
I was the one that allegedly "quit and joined my old band." That wasn't true. But it was said so matter-of-factly on the Internet that the guys weren't really sure what I was up to. Slash
guy spy holy
I once dated a guy who was like, 'Holy sh--, I just made out with Harriet the Spy!' And that's messed up. Don't say that. I was 10, you're 30, it's just weird. Michelle Trachtenberg
guys start
More guys will see the field. It's kind of an audition. You want to play, you start battling. Ron Clarke
guys neither sideline sleepy team watching
Neither team was really controlling the tempo. I think I was getting sleepy on the sideline watching a lot of this game. Our guys were just really tentative. Vic Wolffis
guys neither
Neither one of these guys can put the other guy away. Tom Cole
guys half neil second shoulders sort step took
Neil sort of took us on his shoulders in the first half. The second half we had guys step up with big shots, big plays. John Vaji
guys played pleased rodney
I was really pleased with the way Rodney pitched today. I thought all our guys played hard. Greg Hamilton
guys tough
If we can get those guys healthy, we'll be really tough to beat. George Baker
guys key problem three
I feel real comfortable with our backfield and I think it's going to be a big part of our success. We have three guys that are going to give defenses a real problem if they try to key on one guy. Greg McGirt
guys hard kept key played pressure relentless
I feel like the key was our relentless pressure. We played 12 guys and kept rotating guys in to keep the pressure on. They're a little inexperienced, so we wanted to make it as hard as we could. Kurt Ruth
guys knows takes win
I feel like the guys knows what it takes to win right now. Vince Young
less money people products provide socialists tend work
Socialists tend to want to pay people more money to do less work, and capitalists tend to want to provide better products at better prices. Robert Kiyosaki
less likely turbulent
If you've had a turbulent August/September period, ... It's less likely you're going to get sucker-punched in October. Robert H. Connelly
people time touch
This is the first time when we can touch people when they have been devastated. Tim Cox
people standing union
This is not a union that is struggling. People see we are standing up for what they want and they want to join. Mark Serwotka
people triumph
This is not a triumph for Chirac. People didn't go to vote. Dominique Moisi
people problem turning
This is not about turning people away from the park, ... The problem is not that there's too many people. The problem is there's too many cars. Bruce Babbitt
people
This is not to make it easier, it's just to entice people to come. Julie Taymor
people recession report represents simply
This is simply what a recession is like. This report represents real people and real pain, especially around the holidays. Bill Cheney
people
This is really good. People are being vaccinated. Barbara Schneeman
people season surprising
This is probably the most surprising season, my season and the team's. For a lot of people around the league. Jaromir Jagr
people preserve wonderful
This is so wonderful to see all of these people who are working to preserve our history, Harry Price